The study of mitochondrial sequences integrated in the bovine nuclear genome

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The study of mitochondrial sequences integrated in the bovine nuclear genome

The study of mitochondrial sequences integrated in the bovine nuclear genome

Nuclear copies of mitochondrial DNA (NUMT) have already been described in several species. Dominique Rocha of the G2B team and his partners ientified and analyzed 166 bovine NUMTof a total length of 430 kpb, representing approximately 0,02% of the nuclear genome of cattle. Their studies were published in Scientific Reports.

Copies of all mitochondrial regions were detected in the nuclear genome, with distinct degrees of sequence similarities with the mitogenome. Certain NUMT regions include large mitogenome segments and present great similarities with the organelle genome sequence. NUMT regions are frequently modified by insertion of repeated sequences and by sequence rearrangements. Scientists confirmed the existence of 29 NUMT regions by PCR amplification using cow's DNA (Dominette), which was used to generate the reference sequence of the bovine genome, excluding the possiblity that these NUMT may be artifacts of genome assembly. Since NUMT regions exist with great similarities with the mitogenome, particular attention is necessary during the conception of primers for mitochondrial DNA amplification. These results may therefore serve to avoid the co-amplification of bovine nuclear mitochondria.

 
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See also

Grau, E.T., Charles, M., Féménia, M. et al. Survey of mitochondrial sequences integrated into the bovine nuclear genome. Sci Rep 10, 2077 (2020). https://doi.org/10.1038/s41598-020-59155-4

Modification date : 14 September 2023 | Publication date : 09 April 2020 | Redactor : D. Rocha - Edition P. Huan - Translation W Brand-Williams