Know more

Our use of cookies

Cookies are a set of data stored on a user’s device when the user browses a web site. The data is in a file containing an ID number, the name of the server which deposited it and, in some cases, an expiry date. We use cookies to record information about your visit, language of preference, and other parameters on the site in order to optimise your next visit and make the site even more useful to you.

To improve your experience, we use cookies to store certain browsing information and provide secure navigation, and to collect statistics with a view to improve the site’s features. For a complete list of the cookies we use, download “Ghostery”, a free plug-in for browsers which can detect, and, in some cases, block cookies.

Ghostery is available here for free: https://www.ghostery.com/fr/products/

You can also visit the CNIL web site for instructions on how to configure your browser to manage cookie storage on your device.

In the case of third-party advertising cookies, you can also visit the following site: http://www.youronlinechoices.com/fr/controler-ses-cookies/, offered by digital advertising professionals within the European Digital Advertising Alliance (EDAA). From the site, you can deny or accept the cookies used by advertising professionals who are members.

It is also possible to block certain third-party cookies directly via publishers:

Cookie type

Means of blocking

Analytical and performance cookies

Realytics
Google Analytics
Spoteffects
Optimizely

Targeted advertising cookies

DoubleClick
Mediarithmics

The following types of cookies may be used on our websites:

Mandatory cookies

Functional cookies

Social media and advertising cookies

These cookies are needed to ensure the proper functioning of the site and cannot be disabled. They help ensure a secure connection and the basic availability of our website.

These cookies allow us to analyse site use in order to measure and optimise performance. They allow us to store your sign-in information and display the different components of our website in a more coherent way.

These cookies are used by advertising agencies such as Google and by social media sites such as LinkedIn and Facebook. Among other things, they allow pages to be shared on social media, the posting of comments, and the publication (on our site or elsewhere) of ads that reflect your centres of interest.

Our EZPublish content management system (CMS) uses CAS and PHP session cookies and the New Relic cookie for monitoring purposes (IP, response times).

These cookies are deleted at the end of the browsing session (when you log off or close your browser window)

Our EZPublish content management system (CMS) uses the XiTi cookie to measure traffic. Our service provider is AT Internet. This company stores data (IPs, date and time of access, length of the visit and pages viewed) for six months.

Our EZPublish content management system (CMS) does not use this type of cookie.

For more information about the cookies we use, contact INRA’s Data Protection Officer by email at cil-dpo@inra.fr or by post at:

INRA
24, chemin de Borde Rouge –Auzeville – CS52627
31326 Castanet Tolosan CEDEX - France

Dernière mise à jour : Mai 2018

Menu Logo Principal AgroParisTech Université Paris-Saclay

INRA GABI Unit

GABI : Génétique Animale et Biologie IntégrativeUnité Mixte de Recherche INRA - AgroParisTech

Céline Bourdon will defend her PhD dissertation via video conferencing on Monday March 15 at 9.30

15 March 2021

Réservés
Asociation between microRNA genetic variants and dairy QTL in bovine, caprine and ovine species

Céline Bourdon will defend her PhD dissertation entitled "Association between microRNA genetic variants and dairy QTL in bovine, caprine and ovine species" on Monday March 15 at 9.30 by video conference.

Jury members:

M. Juan José ARRANZ                  University of Leon                        Reporter

Mme Christine GASPIN                INRAE - UMR MIAT                       Reporter

Mme Rozenn DALBIES-TRAN      INRAE - UMR PRC                          Examinator

Mme Christine LEROUX               INRAE - UMR Herbivores             Examinator

M. Bernard MIGNOTTE                Université Paris Saclay                 Examinator

Mme Fabienne LE PROVOST       INRAE – UMR GABI                       PhD Advisor

Mme Gwenola TOSSER-KLOPP   INRAE - UMR GenPhySE               PhD co-Adivsor

Key-words: dairy QTL, microARN, Genetic Variants

Abstract:

Genomic selection, based on the prediction of the genetic value of candidate animals based on the information provided by a large number of genetic markers using neutral markers, is a relevant and perennial lever. The search for causal mutations and their integration into genomic evaluations would allow a significant gain in precision. It is therefore essential to better characterize the causal mutations responsible for the variability of quantitative traits related to production efficiency and the quality of products such as milk. The objective of this project has been to search for genetic variants of microRNA expressed in the mammary gland or present in milk and located in genomic regions having an effect on dairy and mastitis quantitative traits (QTL), in three ruminant species. The detection of 59,124 microRNA variants expressed in the mammary gland or present in milk in cattle, 13,427 variants in goats and 4,761 in sheep has been allowed through the development of a bioinformatics script.    In cattle, 4,679 genetic variants of interest have been located in dairy and mastitis QTLs and 127 in goats, none in sheep. Three detected bovine variants have been validated through GWAS studies. The biological effects of the validated variants have been studied, with different strategies depending on the location and thus the putative effect of the mutation. For the mutation located in the "seed" region of bta-let-7e, the expression level of targeted mRNA has been tested. Inconsistent results were obtained between the qRT-PCR and RNAseq techniques used. For mutations located in the flanking regions of bta-miR-92b and bta-miR-486, the presence of these microRNA has been measured in bovine milk. These analyses did not reveal any significant difference in the expression of microRNA between genotypes. This project therefore has allowed a global analysis of microRNA variants, from their detections to their potential effects.