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24, chemin de Borde Rouge -Auzeville - CS52627 31326 Castanet Tolosan cedex - France

Last update: May 2021

Menu Logo Principal PROSE Université Paris Saclay Carnot 3BCAR

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Olivier Chapleur

Permanent scientist in microbial ecology

Leader of the PROSE analytical division

Olivier ChapleurOlivier Chapleur Wordcloud

Lines of research

  • Methanization of ligno-cellulosic waste and biowaste, inhibition mitigation and process optimisation
  • Molecular ecology approaches (omics, isotope labelling) to study complex microbial communities
  • Non-targeted metabolomics to decipher biodegradation pathways
  • Statistical analysis of data, omics data integration, time-course modelling

Ongoing and past projects

  • STABILICS - ANR project (2019-2023) | Coupling statistics and multi-omics to gain new insights in the determinants of anaerobic microbial bioprocess stability
  • DIGESTOMIC - ANR project (2016-2020) | New operational strategies to overcome technical barriers in anaerobic digestion and extend its fields of application by using meta-omic approaches
  • FASIC PHC project (2017-2018) | Novel statistical methods to improve waste degradation: merging advanced biotechnologies with forefront analytical tools, identification of biomarkers by integrating multiple-omics datasets 
  • METHARESIST - EC2CO project (2015-2016) | Omics approaches to identify resistance strategies of anaerobic digesters to micropollutants : example of phenol

Professional experiences


PhD students
  • Laëtitia Cardona (2017-2020) | "Advancing environmental biotechnologies through molecular ecology approaches: from description to management" 
  • Simon Poirier (2013-2016) | "Anaerobic digestion inhibitions: ecological dynamics, microbial indicators and resistance strategies. Examples of phenol and ammonia nitrogen"
  • Supervision of 17 master students or foreign PhD students during short stays in the lab


Master Degree ECOSEM Ecotechnologies for Sustainability and Environment Management, Ecole polytechnique

Selected publications

  • Assessment of substrate biodegradability improvement in anaerobic Co-digestion using a chemometrics-based metabolomic approach. Puig-Castellví, F., Cardona, L., Jouan-Rimbaud Bouveresse, D., Cordella, C.B.Y., Mazéas, L., Rutledge, D.N., Chapleur, O. (2020). Chemosphere. 254:126812. DOI: 10.1016/j.chemosphere.2020.126812
  • Assessment of the microbial interplay during anaerobic co-digestion of wastewater sludge using common components analysis. Puig-Castellví, F., Cardona, L., Jouan-Rimbaud Bouveresse, D., Cordella, C.B.Y., Mazéas, L., Rutledge, D.N., Chapleur, O. (2020). PLOS ONE, 15:e0232324. DOI: 10.1371/journal.pone.0232324
  • Ecological consequences of abrupt temperature changes in anaerobic digesters. Madigou, C., Lê Cao, K.-A., Bureau, C., Mazéas, L., Déjean, S., Chapleur, O. (2019). Chemical Engineering Journal, 361:266-277. DOI: 10.1016/j.cej.2018.12.003
  • A generic multivariate framework for the integration of microbiome longitudinal studies with other data types. Bodein, A.+, Chapleur, O.+, Droit, A., Lê Cao, K. (2019). Frontiers in Genetics. DOI: 10.3389/fgene.2019.00963
  • Anaerobic digestion of biowaste under extreme ammonia concentration: Identification of key microbial phylotypes. Poirier, S., Desmond-Le Quéméner, E., Madigou, C., Bouchez, T., Chapleur, O. (2016). Bioresource Technology, 207:92-101. DOI: 10.1016/j.biortech.2016.01.124  
  • Asymmetrical response of anaerobic digestion microbiota to temperature changes. Chapleur, O., Mazéas, L., Godon, J.J., Bouchez, T. (2016). Applied Microbiology and Biotechnology, 100:1445-1457. DOI: 10.1007/s00253-015-7046-7
  • Co-inoculating ruminal content neither provides active hydrolytic microbes nor improves methanization of 13C-cellulose in batch digestersChapleur, O., Bize, A., Serain, T., Mazéas, L., Bouchez, T. (2014). FEMS Microbiology Ecology, 87: 616-629. DOI: 10.1111/1574-6941.12249

See also